Comments
Final week update!
This past week I spent my last days at the PERL Lab in Oxford. The days went by faster than usual, and before I knew, it was Friday and I had to say goodbye to everyone. Most people in the lab were away the first three days of the week because they had to go to a conference, so I was left on my own to coordinate and complete all the participant interviews during those days. Human research is very strict, so even though it doesn’t seem like we got many participants, we actually made a lot of progress because the initial stages of the project are usually the most challenging ones. It takes a lot of effort and time to get a project up and running; we first need to find participants that are willing to participate, and then we have to clear them to make sure they are safe to take part in the study. During the summer we interviewed 20 participants, but only 12 people were elegible to take part. The project will need 50 participants to be completed, so it will take quite some time before it’s completely finished. I’m really glad I got to be there during this stage of the project because I learned what’s needed to start a project as well as how to prepare and comply with all the requirements. This summer I also got to help with a Parkinson’s project, and although I didn’t get much hands-on experience, I got to see the different tests that were performed on patients and the behavioral tasks they were given to measure their cognitive functions. Overall, it was a great summer because I not only learned a lot about the psychiatry field, but also about myself. Traveling and living abroad for two months can be scary and overwhelming, but it is totally worth it because it’s an experience that allows you to grow personally and academically. I want to thank LSAMP for the opportunity that they gave me this summer and for giving me the tools to achieve my dreams. I was given the freedom to choose my own lab based on my specific interests, and thanks to this, I enjoyed every day in the lab. I also got the chance to make life long friends and make connections at one of the most renowned universities in the world. It was an amazing summer and I will always be grateful for the opportunity and the support. Finally, I want to give a special thanks to Lynda for all the help. She prepared us for all the situations that we had to face this summer, and without her, this experience wouldn’t have been half as good as it was.
Week 8 update *Long final post*
I was not emotionally ready for my last week at the National Oceanography center. My lab PI’s were so welcoming since the beginning, and I have learned so much from them as individuals and educators all throughout my time here. I teared a bit when I was last thanking them for trusting me with their lab and their discovery collections. I have been so privileged to see and handle all the deep-sea creatures they collect. These animals come from depths at 4000 meters below sea level and are very rare to see in museums (never seen in aquariums). However, I had the grand privilege to touch so many of them, identify them, dissect them, and do DNA extractions from their tissue.
On my final week, I worked on something different concerning specimens. Sea stars had been the only specimens I had not yet done molecular work in, so I dedicated my final days to them. These creatures are rough to dissect because there is no tissue in them at all. I had to carefully take this really thin lining right below their exoskeleton to do molecular work on. At the end, I worked on an excel sheet that summarized my learning experience here and I was really surprised at the results.
Overall, I collected 154 tissue samples using sterile techniques, I did 105 DNA extractions, and I was able to amplify the targeted gene in 23 different samples. In addition, I also curated a collection from 1958 that had 208 amphipods, each of which had to be preserved in its individual way.
My lab Pi’s were really excited along with me because this was a huge step forward in trying to test universal primers that could be used for identifying a clear majority of deep-sea creatures. It is really complicated to just design one set of primers for an entire community, but it is possible to have a couple set of primers that could be used on most deep-sea animals. This would make genetic identification much easier for deep sea creatures, and this was the first stepping stone towards that. The primers we tested were having good success rates, and I hope it continues that way, even if I am not there to see it.
I would like to take the time to thank LSAMP for providing the means for me to do this. These past eight weeks have been so life changing in terms of growth as an individual and student. This experience was a reinforcement of how much I love research and marine science. I am so proud that I can now say I have genetics experience in both, a model organism and animal populations. Both have their pros and cons, but I greatly enjoy doing research in each. Now, more than ever, I am so excited to return to school and continue researching!!! THANK YOU LSAMP! This summer research abroad was such an impactful experience. I’ll leave you guys with one of my favorite quotes: “Remember to look up at the stars and not down at your feet. Try to make sense of what you see and wonder about what makes the universe exist. Be curious. And however difficult life may seem, there is always something you can do and succeed at. It matters that you don't just give up.” – Stephen Hawking.
Week 7 update
Week 7 was almost all about holothurians. First, I collected tissues from Oneirophanta mutabilis and Molpadiodemas villosus.(30 samples total) However, when I tried to do the DNA extraction from the tissues, I ran into an unexpected problem. The tissue would dissolve but when I would transfer it to the filter, the liquid was gooey so it would clog the filter, thus DNA could no longer be obtained. Dr.Rob and I tried to trouble shoot what was wrong with the tissue and hypothesized that the defrosting of the holothurians could have influenced the amount of mucus inside the specimen.(This is why it is so important to keep specimens in ethanol and freezing temperature!) Because of this, Dr.Rob allowed me to use the holothurian samples he had kept in the freezer, which were 20 samples total that included 8 different species. The DNA extractions worked perfectly on these samples!
Aside from that, Dr.Rob taught me how to use a combination of primers to try and amplify a targeted gene. For instance, you can use a universal long primer and a specific species primer together. I tried four different combinations of primers total, & I am waiting on the results.
Lastly, I would like to talk about my weekend, which was pretty busy. Saturday was my birthday, so I went out to the city center and filled myself with so much food that I ended up stomach sick for the rest of the day. I should’ve known my small body cannot intake that amount of food😭Sunday, I tried to make up for the fact I did not fully enjoy my birthday so I took a spontaneous trip to the Isle of Wight. This island is very famous because Queen Victoria used to live here, and Charles Darwin did some of his work in this island.( the island is ranked as the 6th best place in the world for finding dinosaur remains.) I took a 20 minute ferry and arriving there made me so happy because of how beautiful this island is. It really feels like going back in time. Plus, its beach water is sooooo clear and you can view the cliffs from almost every corner. It’s a must visit place!
Week 7 Update
This week I was extremely busy in lab screening a lot of new participants that want to take part in out study. I was able to screen the volunteers and carry the entire interview process on my own, which was a little overwhelming at first but definitely helped me a lot. I also attended a couple talks in the department, in which they discussed topics regarding dementia, the mental health system, and some new computer programs that could help make research much more accesible to everyone. I learned a lot during these talks and it was really interesting to hear about all these topics in a perspective that’s different from the one they are usually discussed back home. I feel like this week, more than learning new things, I got the chance to practice what I’ve been learning the past couple months and polish my technique to deal with participants. I had a very productive week, during which I was able to make a lot of progress in the project as well as have some personal growth.
Outside of lab, I spent my last weekend in Oxford walking around the city center and getting souvenirs for my family and friends. I went back to the library on Saturday and spent most of the day there, trying to absorb all of its beauty and store it in my memory forever! I’m going to miss this place so much, but all I can do is enjoy my last week and make the best out of it! :)
Week 6 update
Before I talk about the research I want to share something I was really excited about doing, which was visiting Stonehenge. This bizarre and interesting monument has existed for almost 5,000 years and it is something I have always watched documentaries on. To some people, they’re just stones. But to me, it is such a god like monument that still has people wondering how it came to be. Standing in front of the stones was such an indescribable feeling! I’m so happy I can say I’ve officially seen the stones❤️
Aside from that, we have had really good success rates with the universal primers we have been trying on deep sea specimens. First, I would like to say that before checking the gel, Dr.Rob taught me how to do PCR cleanup using a vacuum. Basically, the PCR product is added to the top of a filter, then the vacuum sucks up the PCR product through the filter while all DNA is getting attached to the filter. So then you end up with a dry filter to which you then add water, and because of polarity rules, the DNA is released from the filter to the water, and there you have your clean sequence. When we checked the gel, I was really excited because 18 out of the 23 PCR’s I set up worked, so I was able to send of a total of 23 PCR products to be sequenced which had a variety of 5 different species. For the rest of the week we kept collecting tissue from populations. By the end of Friday, I had 66 new tissue samples to start DNA testing on. Within those 66 samples, we had 6 new species we have not tried the primers on so I am really excited to start on that this week. Overall, I was really happy with the new techniques I learned this week, and the success we have had!