06/01/2026
The Haber-Bosch process for reducing N2 gas to ammonia (first developed for producing munitions) requires a temperature of 500C, a pressure of up 300 atmospheres and a catalyst to break that pesky triple bond. Rhizobia do the trick under ambient conditions and for benign purposes. We can learn a lot from microbes.
The traditional acetylene reduction assay — for decades the standard method for measuring nitrogenous activity — has well-documented limitations. Acetylene inhibits the key enzyme involved in N2-fixation by reducing the permeability of a barrier to O2 diffusion in the legume nodule, thus limiting the ATP production required for the energy-expensive reaction.
Direct measurement of H2 evolution, the natural by-product of nitrogenase activity, provides a more reliable method. Our Q-Box NF1LP Nitrogen Fixation Package measures H2 gas evolved by nodulated legume roots directly. The method used by Qubit scientists has shown that published rates of in vivo nitrogenous activity can be underestimated by more than 80%—something of a large error. Learn more here: https://qubitsystems.com/products/plants-algae-soil/nitrogen-fixation/q-box-nf1lp-nitrogen-fixation/
Visit us at the North American Symbiotic Nitrogen Fixation Conference at Queen’s University, Kingston, On June 1st to 4th.